Abstract
Herbicide-resistant Palmer amaranth has been problematic within the United States for the past 30 years. The recent introduction of Palmer amaranth into the Pacific Northwest (PNW) prompted extensive surveys in 2023 and 2024 to collect seed samples for herbicide-resistance screening and leaf tissue for resistance-mechanism genotyping. Greenhouse dose-response bioassays were conducted in Kimberly, ID, during the summer of 2024 to assess the response of Palmer amaranth populations to selected postemergence herbicides. Resistance to glyphosate predominated across populations, and reduced sensitivity to 2,4-D, dicamba, and mesotrione was also observed. In contrast, glufosinate and saflufenacil provided effective control of PNW Palmer amaranth populations. Based on the dose-response bioassays, the effective dose required to provide 90% control (ED 90 ) of the suspected glyphosate-resistant populations was 20 to 63-fold compared to the susceptible population. Subsequent 5-enolpyruvylshikimate-3-phosphate synthase ( EPSPS ) gene duplication analysis was conducted to confirm glyphosate resistance in the Palmer amaranth populations. About 74% (17 of 23) of the Palmer amaranth tissue samples showed gene duplication, with up to 150 copies of the EPSPS gene. The EPSPS gene amplification analysis of plants that survived 2X rate of glyphosate (2,520 g ae ha -1 ) showed up to 150 EPSPS genes in glyphosate-resistant populations. The widespread glyphosate resistance in the collected samples suggests that Palmer amaranth populations are being introduced into the PNW from locations where resistance to herbicide sites of action has previously evolved.