Abstract
The use of an ammonia fiber expansion pretreatment using low-moisture anhydrous ammonia (LMAA) is a promising strategy for biomass deconstruction, with significant effects on depolymerizing lignin and hemicellulose. An LMAA pretreatment provides several advantages, including compatibility with the high-biomass loading of solids, efficient ammonia recovery, and scalability for industrial operations. In this study, the reactor was revisited and optimized to improve glucan digestibility from corn stover through enzymatic hydrolysis, building on our previous findings that identified limitations in ammonia distribution. The effects of the biomass particle size, the reaction time, and their interaction on glucose yields were investigated to determine their influence on the subsequent enzymatic hydrolysis kinetics. The best glucose yield of 83% was achieved using an LMAA pretreatment of biomass with a 0.5 mm particle size, representing an improvement of approximately 5% compared to biomass with a 1 mm particle size. Additionally, reactor optimization led to a 22% improvement in the glucose yield compared to the previous reactor configuration. According to the results of the reaction kinetics fitting, the enzymatic hydrolysis data indicated that the reaction followed a pseudo-first-order model.