Abstract
Artificial insemination (AI) is an effective strategy to distribute semen from elite sires and improve the genetics of a herd. Commercial studs provide a fertile semen product to their customers. Consequently, the maintenance of sire fertility is dependent upon appropriate semen storage, handing, and site of semen deposition. When retrieving a straw of frozen semen, the cane (and canister containing canes) should be held below the frost-line and limited to 8 seconds in the neck of the tank. After appropriate thawing, and continuous thermoregulation of the AI gun containing the thawed semen, semen should be deposited in the uterine body.