Abstract
The recent paper by Root-Bernstein & Bernstein (2024) in The Journal of Physiology on the E-poker modelling of Lenski's Long-Term Evolution Experiment (LTEE) cites our work (van Hofwegen et al., 2016). We appreciate the insightful exploration by the authors, especially regarding the role of interactomes and evolutionary processes. We would like to clarify several points that refer to our findings and add context to the general discussion about evolutionary contingency and interpreting the LTEE Escherichia coli Cit+ phenotype.