Efficient and specific DNA-targeting using single-stranded LNA/MOE mixmers and chimeric Invader:XenoRNA probes

Michaela E Everly; Patrick J Hrdlicka

doi:10.1039/d5ob01570g

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Efficient and specific DNA-targeting using single-stranded LNA/MOE mixmers and chimeric Invader:XenoRNA probes

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Efficient and specific DNA-targeting using single-stranded LNA/MOE mixmers and chimeric Invader:XenoRNA probes

Michaela E Everly and Patrick J Hrdlicka

Organic & biomolecular chemistry

10/20/2025

DOI: https://doi.org/10.1039/d5ob01570g

PMID: 41114820

Abstract

DNA-targeting properties of heteroduplexes between intercalator-functionalized oligonucleotides and various Xeno RNAs ( , LNA/MOE mixmers, or fully modified MOE, O2'-Me, or 2'-F strands) are disclosed. Chimeric Invader:LNA/MOE probes and some single-stranded LNA/MOE mixmers enable efficient invasion of double-stranded DNA targets with exceptional binding specificity.

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Title: Efficient and specific DNA-targeting using single-stranded LNA/MOE mixmers and chimeric Invader:XenoRNA probes
Creators: Michaela E Everly - Department of Chemistry, University of Idaho, Moscow, Idaho 83844-2343, USA. hrdlicka@uidaho.edu
Patrick J Hrdlicka - Department of Chemistry, University of Idaho, Moscow, Idaho 83844-2343, USA. hrdlicka@uidaho.edu
Publication Details: Organic & biomolecular chemistry
Publisher: Royal Society of Chemistry
Identifiers: 996844123101851
Academic Unit: Chemistry; Initiative for Bioinformatics and Evolutionary Studies
Language: English
Resource Type: Journal article