Abstract
This folder contains genotyping data for Bromus tectorum accessions collected throughout the PNW small grain production region. Data was obtained using a reduced representation genotype-by-sequencing (GBS) approach to identifying SNP molecular markers. The GBS approach used in relied upon a combination of a regular and a methylation-sensitive restriction enzyme to avoid repetitive regions of a target genome and instead favor lower copy regions to increase the probability of identifying informative genetic markers. A two-enzyme approach originally developed for species lacking a reference genome was utilized, where a rare (Pst1, CTGCA^G) and common (Msp1, C^CGG) restriction enzyme were used in combination. A modified GBS protocol developed by Mascher et al. (2013) for use with semi-conductor sequencing platform was followed according to protocol. The resulting amplicons were sequenced on an Ion Proton sequencer using an Ion P1 Chip (Life Technologies, Carlsbad, CA). The master FASTQ file obtained from the sequencer was parsed by barcode to separate each accession into a separate file. FASTQ file size ranged from 5 to 112 MBs with an average size of 45.6 MBs
The data files associated with this record are no longer actively supported from this repository, please contact original data creator if you need to access these data.